Genetic Divergence of Two Vietnamese Swamp Buffalo Populations using Microsatellite Markers
Genetic Divergence of Two Vietnamese Swamp Buffalo Populations using Microsatellite Markers
Van Dai Nguyen1, ¥, Van Ba Nguyen2, ¥, Thi Thanh Nhan Giang2, Cong Dinh Nguyen2, Thi Lan Nguyen1, Dinh Ngoan Vu1, Duc Chuyen Nguyen1, Van Can Ta1, Khac Khanh Nguyen2, Thi Lien Cao2, Doan Lan Pham2, Khanh Van Nguyen2, Ngoc Tan Nguyen3*
ABSTRACT
This study evaluated the genetic diversity of two Vietnamese swamp buffalo populations, LangBiang (LB) in Lam Dong and Thanh Chuong (TC) in Nghe An Provinces of Vietnam. Ear tissue samples were collected from 96 buffaloes including 64 LB (32 LB-LD and 32 LB-LC) and 32 TC and a set of 18 microsatellite markers was used to analyze their genetic diversity. Results showed that all the microsatellite markers were polymorphic in the two local swamp buffalo populations. The average number of alleles of each microsatellite marker in the two native buffalo populations varied from 6.11 (in LB-LD and LB-LC sub-populations) to 7.67 (in the TC population), with mean values of expected heterozygosity (He), observed heterozygosity (Ho), and polymorphic informative content (PIC) 0.743, 0.271, and 0.738, respectively. The two local swamp buffalo populations were separated into two main branches. One branch came from the TC local buffalo population while the other from the LB buffalo population subsequently separated into two sub-clades (LB-LD and LB-LC). Most of the polymorphic markers used in this study were applicable for investigating the genetic relationships among Vietnamese local swamp buffalo populations. Inbreeding among the two Vietnamese native buffalo populations was indicated, the separation of PB into the two LB sub-populations and bull exchange policy among populations requires further study.
Keywords | Bubalus bubalis, Genetic divergence, Native buffalo, Microsatellite marker, Polymorphism, Swamp buffalo
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