Evaluation of Chemo-Micobial Methods for Cellulose Hydrolysis in Sugarcane Bagasse and Saw Dust
Evaluation of Chemo-Micobial Methods for Cellulose Hydrolysis in Sugarcane Bagasse and Saw Dust
Samra Aftab1, Saleem Ullah1 and Farida Anjum2*
ABSTRACT
Saw dust and sugarcane bagasse were treated with acids/bases, microbial cultures and their enzyme extracts to check their effect on cellulose hydrolysis. Microbes were isolated from soil by using serial dilution method and were selected on efficiency basis (screening of microbes on the basis of their activities as cellulose degrading agents). The cellulose and related constituent of saw dust and sugarcane bagasse showed that cellulose (49 %), hemicelluloses (23.5 %) and ash (1.8 %) were higher in sugarcane bagasse, while lignin (7.9 %) was higher in saw dust. The data regarding microbe isolation showed highest colonies of Aspergillus sp. (8 CFU × 104g-1) followed by Penicillium sp. and Trichoderma sp. (7 CFU × 104g-1 and 5 CFU × 104g-1), while the lowest colonies were noted for Candida ablican and Saccharomyces cerevisiae. The screening test of these microbes for cellulose degradation showed higher degradation for Trichoderma sp. (6 % and 8 %) followed by Penicillium sp. (4 % and 4.5 %) in both of the samples (saw dust and sugarcane bagasse) respectively. Similarly the combined effect of acids, bases and microbes showed higher result of NaOH, Trichoderma sp. (29.5 % and 41 %) in both samples (saw dust and sugarcane bagasse). The optimization of incubation time and temperature showed 72 hrs of incubation time and tempreture of 50 °C was best for cellulose degradation. From the data, it was concluded that Trichoderma sp. among microbes, HCl in acid and NaOH in bases were best for cellulose hydrolysis in samples while, incubation of 72 hrs at 50 °C showed higher cellulose degradation rate.
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