Assessment of Oil Vaccination Protection to Newcastle Disease Virus Challenges in Layer
Assessment of Oil Vaccination Protection to Newcastle Disease Virus Challenges in Layer
Qayssar Obaid Muhammed, Raed Hussain Salih Rabee*
ABSTRACT
The study sought to examine the immunological response of layer hens to a novel strategy of immunization employing an inactivated oil-emulsion of Newcastle disease virus vaccine administered through fine spray. The study also examined the long-lasting nature of passive antibodies in layer hens and evaluated their effectiveness in providing protection against different strains of viruses. There were 200 Lohmann Brown chicks, all 1 day old, which were randomly divided into four groups, each containing 50 chicks. Groups 1, 2, and 3 were administered the vaccination via subcutaneous injection by three distinct commercial businesses at the ages of 8, 70, and 115 days, respectively. Additionally, they were also administered the vaccination through a spray administration on the same day. The control group did not get any vaccinations. Chicken specimens were collected 20 days after immunization, at ages of 30, 50, 70, 90, 110, 130, 150, 170, and after challenging at 200 days. The most pronounced levels of hemagglutination inhibition and ELIZA were observed following the administration of the booster dosages. The p-value over 0.05 indicates that there was no notable difference in antibody production among layer chickens. The study demonstrated that the implemented approach effectively guaranteed the well-being of the herd, leading to optimal productivity without any issues or deaths. Furthermore, the presence of maternal antibodies might be observed till the age of 5 days, suggesting the extent of immunity established before vaccination. Upon conducting a challenge with NDV, the results demonstrated a noteworthy rise in HI and ELIZA titers in the G1 group as compared to G2, G3, and the control group, respectively. In addition, the mortality rate was shown to be 20%, 40%, 50%, and 70% in the G1, G2, G3, and control groups, respectively. In conclusion, the humoral immunity, as indicated by the HI and IgG ELIZA tests, demonstrated that the G1 group, which was vaccinated with the MSD® oil killed vaccine, exhibited the most effective immunization compared to the G2, G3, and control groups, respectively, following exposure to NDV.
Keywords | Vaccination, Newcastle disease, oil vaccine, layers and ELIZA
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November 2024
Vol. 12, Sp. Iss. 1
