Microsatellites and Morphometric Diversity Among Indonesian Local Duck Populations in Lombok Island
Microsatellites and Morphometric Diversity Among Indonesian Local Duck Populations in Lombok Island
Maskur Maskur1*, Muhammad Muhsinin1, Lestari, Tapaul Rozi1, Asnawi2
ABSTRACT
The genetic diversity of three breeds of Indonesian local duck in Lombok island West Nusa Tenggara province locus was investigated based on morphometric traits and four microsatellites marker. Experimental ducks consisted of 75 Sasak ducks, 75 Khaki Campbell ducks and 70 Mojosari ducks. Morphometric traits were recorded and statistically analyzed, whereas genetic parameters of microsatellite loci were calculated using PopGene32. In this study, the mean PIC of the 4 microsatellite loci in all populations was 0.472, whereas AY287 was the most polymorphic locus with a PIC value of 0.735 and APL579 was a monomorphic locus with a PIC value of 0 (zero). The mean value of observed heterozygosity (Ho) is lower than expected (He), with the values 0.393 and 0.472 (Khaki Campbell); 0.433 and 0.532 (Mojosari); and 0.330 and 0.451 (Sasak), respectively. This indicates that the population is heading towards a heterozygosity deficit and proves that the three populations are not in Hardy-Weinberg equilibrium. The inbreeding coefficient in the three populations was quite high with a positive Fis value ranging from 0.000 – 0.422. Meanwhile, the value of the genetic differentiation index (Fst) in all populations is zero (0), indicating that there is no differentiation between breeds at the observed loci.
Keywords | Local duck, Microsatellites, Morphometric, Inbreeding and differentiation
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