Molecular Detection of mecA Gene in Staphylococcus sp. Associated with Ruminant Mastitis
Molecular Detection of mecA Gene in Staphylococcus sp. Associated with Ruminant Mastitis
Hanan Yousif Jasim1*, Rasha Munther Othman2, Wasan Moaed Shaker3
ABSTRACT
This study was aimed to detection the distribution of mecA gene among Staphylococcus sp. Isolated from mastitis cows, sheep and goats in Basrah/Iraq. A total of 26/70 (37.14%) was primarily isolated on fermented Mannitol salt agar (MSA) and examined by Gram stain. All suspected Staphylococcus sp. isolates were further detection by PCR and analysis the partial sequencing of the 16s rDNA gene and compared with those in the GenBank to find variances in the sequence using the BLAST tool (http://www.ncbi.nlm.nih.gov). The sequencing results of the 16s rDNA gene was shown that 9 (69.24%) bacterial isolates were diagnosed as Staphylococcus aureus while the 4 (30.76%) other samples were diagnosed as Staphylococcus sciuri, Staphylococcus succinus, Staphylococcus equorum and Staphylococcus epidermidis. On the other hand, the mecA gene was examined and detected in all isolates of Staphylococcus sp. using specific primer, and the sequence analysis results of this gene revealed that 11 isolates shown 100% homology to the Staphylococcus aureus cassette chromosome mec with ID number LC727174.1 and Staphylococcus aureus strain C249 chromosome with ID number CP127807.1. While one isolate was displayed 100% homology to the mecA gene that previously detected in Staphylococcus epidermidis strain 1FSE05 plasmid with ID: CP121525.1. We concluded that all this information may offer crucial understanding of the origin and persistence of current and possibly even future of mec A gene in Staphylococcus strains.
Keywords | Staphylococcus sp., mecA gene, Staphylococcus aureus, MRSA
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July 2024
Vol. 12, Iss. 7, pp. 1206-1409
