Synthesis, Characterization and Investigation of the Anticoccidial Activity of New formulation Curcumin - Olive Oil Nano-Composite
Synthesis, Characterization and Investigation of the Anticoccidial Activity of New formulation Curcumin - Olive Oil Nano-Composite
Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1*
ABSTRACT
New formulation of nanocurcumin (curcumin-olive oil nano-composite; C-Oo.Nc) was produced and characterized in the present study. The produced nanoparticles are spherical with 63 nm zeta size and high stability in the aqueous media. The coccidiocidal efficacy of the C-Oo.Nc was evaluated versus five chicken Eimeria species oocysts. The immature oocysts were exposed to 5, 10, 20, and 40 ppm, each for 1, 3, 6, 9, 12, 24, and 36 h. Percentage of sporulation inhibition in oocysts and comet assay were used to investigate the C-Oo.Nc destructive effects. The infectivity of sporulated oocysts exposed to LC50 & LC90 were evaluated by In vivo inoculation of broiler chicks. The results demonstrated that the increase in the rate of sporulation inhibition is directly related to the increase in C-Oo.Nc concentrations and exposure time. Analyzing the DNA damage in the oocysts exposed to different concentrations using comet assay revealed a significant direct variation (p ≤ .05) between the increase in the dose and the degree of the DNA genotoxic damage as represented by variations in the tail length (μm), percent of DNA in the tail segment, and tail moment (μm). Postmortem (PM) inspection of birds inoculated by LC90 exposed sporulated oocysts demonstrated a very slight or un-apparent reddens without thickening in the cecal wall with close relation to the normal un-infected control group. This with in contrast to the group inoculated by LC50 exposed sporulated oocysts. The study concluded that C-Oo.Nc is a promising effective anticoccidial compound for controlling infection in chickens.
Keywords | Curcumin -olive oil nanocomposite, anticoccidial activity, Comet assay, Poultry, coccidiosis.
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