Multi-Locus Genetic Analysis in Silver Carp Populations of Indus River as Influenced by Restocking Programs
Multi-Locus Genetic Analysis in Silver Carp Populations of Indus River as Influenced by Restocking Programs
Taqwa Safdar, Khalid Abbas*, Muhammad Sarfraz Ahmed, Hina Amjad, Sumra Naz and Jamal Kazam
ABSTRACT
The genetic integrity of fish populations has been continuously threatened due to various anthropogenic actions, particularly restocking programs. These losses in genetic diversity present erosion of global biodiversity. The genetic status of Hypophthalmichthys molitrix in the Indus River was assessed by using microsatellite markers. For the purpose, samples were collected from five selective Indus River sites, and DNA extraction was done by proteinase-K and standard phenol/chloroform DNA isolation method. Genomic DNA was PCR amplified by Labeo rohita cross-species amplification of H. molitrix. The results showed a low-to-moderate level of genetic diversity. The number of alleles on each locus ranging from 2.0 to 6.0, with an average of 3.48 was observed at various loci. The average observed and expected heterozygosities ranged from 0.664 to 0.76 and 0.631 to 0.664, respectively. For all tested loci, population combinations showed significant deviation (p<0.05) from HWE. The AMOVA indicated that most of the variation lied within individuals in populations of H. molitrix. Based on Nei’s genetic distance, UPGMA dendrogram was constructed that resulted in two clusters: one cluster consisted of JB and CB while other consisted of GG, GB and TB. The findings of this research will be useful for restoring, conservation and monitoring the natural aquatic fish species in Pakistan.
To share on other social networks, click on any share button. What are these?
