Genetic Diversity and Genotyping of Canine Parvovirus Type 2 by Using the Full-Length Vp2 Gene in North Iraq
Genetic Diversity and Genotyping of Canine Parvovirus Type 2 by Using the Full-Length Vp2 Gene in North Iraq
Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4
ABSTRACT
Canine parvovirus-2 (CPV-2) has been considered to be a significant pathogen of domestic and wild canids and has spread worldwide. This study was conducted on fecal samples from suspected parvovirus dogs were taken from different provinces in north Iraq for PCR testing, and 76% of such samples were confirmed to be positive. Sequence analysis showed two CPV-2c, six new CPV-2b, and ten new CPV-2a variants. In the CPV-2 studied, several unique and existing mutations have been discovered, indicating the emergence of CPV-2c variation in Iraq. Genetic differentiation study based on the genetic variation on the VP2 gene has shown that (CPV-2/Sul) strain belongs to new CPV-2b and CPV-2c, however, the (CPV-2/Erbil & CPV-2/krk) strains belong to new CPV-2a. A Phylogenetic tree constructed basis on the VP2 gene revealed that the field virus sequences from the present study were divided into two main phylogenetic groups (cluster A and cluster B), 10 new CPV-2a from a field isolate belonging to cluster A with (china-MF423125) and (Italian-MK348096) strains with high identities 99.20%-99.70%. The cluster B are contained 5/2 CPV-2b/c of a field isolate with (Thailand-MH711902) and (china-MG013488) strains with high identities range of 98.80%-99.70. The current study on the molecular characterization of CPV-2 will provide foundations to carry out effective control strategies in the future.
Keywords | VP2 gene, Molecular genotyping, Phylogenetic analysis, Viral evolution.
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