Potential Role of Recombinant Capsid Protein of Dengue Virus Serotype 2 in the Development of an In-House Elisa Based Detection of Infection
Potential Role of Recombinant Capsid Protein of Dengue Virus Serotype 2 in the Development of an In-House Elisa Based Detection of Infection
Rakhtasha Munir1, Shazia Rafique2*, Iram Amin1,2, Sameen Ahmed2, Ayesha Vajeeha2, Amjad Ali3, Muhammad Shahid2, Muhammad Umer Khan4 and Muhammad Idrees2
ABSTRACT
Dengue is an arboviral infection predominant in tropical and subtropical areas. It has inhabited the earth for the last few decades affecting almost 50% of the population in developing nations. Dengue infection covers a high range of symptoms for instance mild to acute hemorrhage fever, dengue shock syndrome, and liver dysfunction. Early diagnosis of the disease is crucial for prompt treatment strategies. One of the detection strategies of the infection can be made through viral structural peptides that act as markers for discernment, including Capsid (C) protein. In the proposed research, the structural gene obtained from local isolates was targeted for detection. For this purpose, a bacterial expression system was used to amplify, clone, and express the structural protein C. The expression of structural protein C was examined by SDS PAGE and verified by western blot and dot blot. The acquired antigen was purified which carries the potential for the development of a vaccine against dengue as well as for an in-house diagnostic assay. In this study, an in-house diagnostic ELISA has been compared to a commercially available kit to determine the specificity and sensitivity of the designed diagnostic ELISA. The aim underlying this study was to develop an accurate, error-free, unambiguous, and cost-friendly in-house ELISA, for the detection of antibodies in Pakistan’s most prevalent dengue virus serotype 2 (DENV-2). The progress of this research would likewise foreshadow advancements of novel vaccines for future counteraction of dengue infection as C protein is highly immunogenic.
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