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Prevalence of Mycoplasma gallisepticum in Poultry and Wild Life Birds Suspected of Chronic Respiratory Disease in Northern Pakistan

Prevalence of Mycoplasma gallisepticum in Poultry and Wild Life Birds Suspected of Chronic Respiratory Disease in Northern Pakistan

Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*

1Department of Microbiology, Hazara University, Mansehra 21300, Khyber Pakhtunkhwa
2Department of Animal Nutrition, The University of Agriculture, Peshawar
3Department of Biosciences, Bioscience Block, Chak Shehzad Campus, Park Road, COMSATS Institute of Information Technology, Islamabad
4College of Veterinary Sciences and AH, Section Microbiology, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa
5College of Veterinary Sciences, Bahauddin Zakariya University, Sub Campus Layyah

*      Corresponding authors:;




Seroprevalence and involvement of Mycoplasma gallisepticum (MG) in cases of chronic respiratory disease (CRD) in poultry and pheasantry birds of Northern Pakistan is underreported. In this study we report on the seroprevalence and isolation of MG from a total of 2341 poultry and 24 pheasantry birds. Overall, seropositivity of 46.56% (1090/2341) and 27.2% (6/24) against MG was observed among poultry- and pheasantry- birds, respectively. Broiler breeder and broiler showed 58.93% (264/448) and 37.23% (542/1456) seropositivity, respectively. A total of 17.1% (402/2341) samples collected from all these CRD-suspected birds were found positive on modified fray’s media indicating typical mycoplasma like colonies with appearance of fried egg or nipple like characteristic of 0.1 to 1 mm in diameter with a dense raised center in the middle. Interestingly, culture positivity for broiler samples was found higher (36.8%) as compared to breeders (33.5%) and layers (29.5%), respectively. Overall, of the cultured samples, 71.19% (215/302) were confirmed as M. gallisepticum by specie specific PCR. Furthermore, 82.43% of the confirmed isolates were recovered from Haripur, 82.35% recovered from Abbotabad and 60.53% were recovered from Mansehra. Finally, no significant difference (P<0.05) in PCR based positivity was found among all three types of bird population. Altogether, the observed high seropositivity and concomitant isolation of MG from CRD suspected birds investigated in the Northern region of Pakistan strongly suggest to initiate a comprehensive effective surveillance programme.

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Pakistan Journal of Zoology


Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500


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