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Effects of Rice Growth Stages on the Composition and Functional Potentialities of Rhizosphere Bacterial Community in Coupled Rice-Crab System

Effects of Rice Growth Stages on the Composition and Functional Potentialities of Rhizosphere Bacterial Community in Coupled Rice-Crab System

Yu Song1*, Yueping Wei2 and Peng Wang3

1Yalu River Basin Research Institute, Liaodong University, Dandong 118003, Liaoning, China
2Innovation and Entrepreneurship Center, Liaodong University, Dandong 118003, Liaoning, China
3School of Agriculture, Liaodong University, Dandong 118003, Liaoning, China
 
* Corresponding author: songyu_71@163.com

ABSTRACT

Co-culture of rice with aquatic animals has garnered widespread interest for its potential to control soil-borne diseases, improve soil quality and increase rice yields. Therefore, it is necessary to further explore its mechanism. In this study, the samples of different growth stages of rice, including tillering stage (TS), jointing stage (JS), heading stage (HS) and mature stage (MS) were collected to analyze the impact on the community composition, diversity and functional potentialities of the rhizosphere bacterial in the coupled rice-crab system were investigated through 16S rRNA gene high-throughput sequencing. The results showed that, a total of 444,882 sequences from 12 samples were obtained, and 6637 bacterial operational taxonomic units (OTUs) with 97% sequence similarity were identified. The rhizosphere bacterial communities were more influenced by growth stages of rice than chemical properties. Principal component analysis (PCA) revealed the similarity of samples at four growth stages was significantly low (P <0.05). The dominant taxa phyla for bacterial community were similar in different growth stages. The LefSe analysis indicated that 32 distinctly taxa that were abundant among four stages with a linear discriminant analysis (LDA) score higher than 3.5. The α-diversity of the bacterial community in rhizosphere soil was not significantly different among different growth stages (P >0.05). Moreover, 8 functional potentials among 46 KEGG pathways showed notable differences for the rhizosphere bacterial community and HS had the largest number of species and strongest metabolic function potentials. Overall, the findings in this study provide valuable information for maintaining soil ecosystem balance and provide theoretical guidance for the practical application of this co-culture system.

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Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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