Purification, Serology and Prevalence of Broad bean stain Comovirus (BBSV) and Cowpea Aphid Borne Mosaic Potyvirus (CABMV)
Purification, Serology and Prevalence of Broad bean stain Comovirus (BBSV) and Cowpea Aphid Borne Mosaic Potyvirus (CABMV)
S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy*
ABSTRACT
BBSV and CABMV were purified from faba bean and cowpea plants, respectively using two different methods. The UV absorption spectrum had maxima and minima at 260 and245 nm, respectively for the two viruses. The absorbance ratios of A and A260/280 of the combined lower bands of purified BBSV preparations were 1.19 and 1.62, respectively. Infected faba bean plants yielded up to 0.48 mg/100g tissue. The corresponding data for CABMV were 1.20&1.22 and its yield was 3.52 mg/100g of cowpea leaves. The maximum antiserum titer against either BBSV or CABMV was I :512. The dilution end point of BBSV in faba bean sap was I :320 using indirect ELISA, whereas it was 1:280 for CABMV. Both of BBSV and CABMV were detected in stems and all flower parts (sepals, petals, pistils and anthers) in intact seeds and all seed parts (seed coats, cotyledons and embryos) of immature seeds obtained from infected faba been and cowpea plants. Both of the viruses were not detected in seed coat of ripened seeds and in roots of infected plants. BBSV was detected in stained seeds more than un-stained seeds obtained from infected faba bean plants.
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