Effect of nickel on biofilm formation of Halophilic bacteria isolated from Achyranthus aspera from salt range Pakistan
Effect of nickel on biofilm formation of Halophilic bacteria isolated from Achyranthus aspera from salt range Pakistan
Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*
ABSTRACT
When exposed to heavy metals or toxic compounds, bacteria have multiple survival strategies involving several mechanisms of resistance and tolerance. Biofilm formation is one such resistance mechanism for bacterial survival. This paper provides the basis for use of bacteria isolated from saline environment and to check their biofilm forming ability under nickel stress. Our previous results showed that these bacteria are capable of producing proline, glycine betaine, choline and other osmolytes under NaCl stress. On the basis of those results it is hypothesized that this osmolyte production ability may help them in nickel resistance under Ni salt stress. This study was centered on assessing the adherence, motility, hydrophobicity, aggregation and biofilm formation of two salt tolerant strains [Bacillus subtilis and Halomonas aquamarina (1)] in the presence of nickel. Two concentrations of nickel were used i.e., 500μg/ml and 1000μg/ml for subsequent tests. The results revealed that at low/ sub-inhibitory concentration of nickel (500 μg/ml), motility is inhibited; adhesion, hydrophobicity, aggregation, EPS production and biofilm formation of two strains are increased. This biofilm forming ability can be employed in
urification of waste waters and treating contaminated soils.
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