Preliminary Assessment of Stock Enhancement in Swimming Crab (Portunus trituberculatus) Based on Molecular Markers
Preliminary Assessment of Stock Enhancement in Swimming Crab (Portunus trituberculatus) Based on Molecular Markers
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
ABSTRACT
Assessment of the stock enhancement programs is crucial for fishery resources recovery, yet lack of proper methods hinders the precision and accuracy of such assessment. The swimming crab, Portunus trituberculatus is a commercially important species in Chinese fishery industry. However the natural resources of swimming crabs are declining and enhancement programs are being conducted for resources restoration for decades. In this study, 524 female broodstock from 10 hatcheries and 547 recaptured crabs from 6 investigations were used to assess the proportion of released individuals and evaluate the effect of the program in Shandong Province in 2014. Parentage determination between broodstock and recaptured individuals was implemented by using mitochondrial control region fragments and three microsatellite markers. When using mtDNA loci, 242 individuals (44.24%) were excluded for no shared haplotype with broodstock. Further 81 (14.81%) crabs were identified as hatchery-reared individuals based on microsatellite loci from the remaining 305 crabs. Our results also showed high genetic diversity and a certain degree of heterozygote deficiency of natural swimming crab populations. The advancing technology and its unique advantages will make molecular markers as novel and highly-efficient approaches for assessment of stock enhancement.
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February 2020
Vol. 52, Iss. 1, Pages 1-424
