Interaction Between Rhizobium Meliloti and Its Specific Viruses in Clover Plants
Interaction Between Rhizobium Meliloti and Its Specific Viruses in Clover Plants
B. A. Othman; Kh. A. El-Dougdoug; M. H. Abdel Ghaffar and T. F. El-Arabi
ABSTRACT
Four phages singed as RM1, RM2, RM3, and RM4 specific for Rhizobium meliloti (R meliloti) were isolated from tested rhizosphere soil samples collected from Qalyubia and North Sinai Governorates based on plaque morphology, The extinction spectra of purified preparations showed A260/A280 of 1.25. 1.16. 1.28 and 1.24 for RM1, RM2, RM3, and RM4, respectively. When the purified preparations of RM1, RM2, and RM3 phages examined in transmission electron microscope they showed particles have isometric heads or about 52.5, 58.8 and 64.2 nm in diameter with long contractile tails of about 100x30, 80.2x29.4, and 102x33.5 nm, respectively. While the RM4 phage had an isometric head of about 37.5 nm in diameter with short tail of about 12.5x 5 nm. SDS-PAGE indicated that the phage RM1 contained 6 structural proteins of 62.4, 60. 53.4, 50, 28, and 20 kDa; RM2 had 5 structural proteins of 60.4. 53.4, 50, 21.5 and 20 kDa: RM3 had 4 structural proteins of 60.4, 53.4, 50, and 21 KDa while RM4 had 3 structural proteins or 60.6, 53.4, and 50 kDa. The thermal inactivation points for the four phages were 68, 76, 80, and 64oC for the four phages respectively. Data also showed that the isolated phages have a suppression effects on the role or R. meliloti strains Barseem (parental) and Barseem (mutant), and led to decrease the growth characters and nitrogen content of clover plants. While Barseem IP2 (mutant) was not affected by phages since the growth characters and nitrogen content of clover plants were increased.
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