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Regulatory Effect of Angelica sinensis Polysaccharide on BMP-7/Smads/TGF-β1 Signal Pathway in Kidney of Rats with Radiation Injury

Regulatory Effect of Angelica sinensis Polysaccharide on BMP-7/Smads/TGF-β1 Signal Pathway in Kidney of Rats with Radiation Injury

Xiaowei Huang1, Jinji Wang1, Zhun Yu2, Minghua Duan1* and Zhe Lin1*

1School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
2The Third Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130117, China
 
* Corresponding author: duanduan-2007@163.com, linzhe1228@163.com

ABSTRACT

Angelica sinensis polysaccharide (ASP) is a biomacromolecule that isolated from the roots of Angelica sinensis. This study aims to investigate its protective effect on kidney injury and its influence on BMP-7/Smads/TGF-β1 signal pathway in irradiated rats. Total 60 Sprague Dawley (SD) rats were randomly divided into 5 groups: the normal (normal saline), model (normal saline), and low, medium, high dose of ASP groups (9.0, 18.0 and 36.0 mg/mL, 2.0 mL/kg·d, intragastric gavage once a day for 14 days). On the 15th day, all other groups received 60Co γ-ray irradiation with a total dose of 4.0 Gy except the normal group. The levels of NO synthase (NOS) and NO in serum, the contents of malondialdehyde (MDA) and superoxide dismutase (SOD) in kidney of each group were detected with ELISA after 24 h of irradiation, and the protein expression levels of TGF-β1, phosphorylated (p-)Smad2, p-Smad2, p-Smad1, p-Smad5 and BMP7 in kidney were detected by western blotting. In the results, compared with the model group, NOS, NO and MDA contents were decreased in the middle and high dose groups while SOD contents were increased in low, middle and high dose groups. The levels of TGF-β, p-Smad2 and p-Smad3 were increased in low, middle and high dose groups while the levels of BMP7, p-Smad1 and p-Smad5 were decreased in middle and high dose groups. In conclusion, ASP can reduce the expression levels of TGF-β, p-Smad2 and p-Smad3 in kidney of rats induced by radiation, increase the expression levels of BMP7, p-Smad1 and p-Smad5, and resist the body injury caused by radiation by regulating BMP-7/Smads/TGF-β1 signal pathway.

 

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Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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