Effect of Inorganic Selenium on Blood Biochemistry under Dexamethasone Induced Stress in Broiler Chicken
Effect of Inorganic Selenium on Blood Biochemistry under Dexamethasone Induced Stress in Broiler Chicken
Irfan Ullah1, Asad Ullah2*, Tahira Tayyeb1, Rafiq Ullah1, Muhammad Hanif1, Faiza Khan3, Imad Khan2, Raheela Taj4, Fatima Syed4, Shumaila Gul5, Muhammad Sadeeq6, Muneeb Islam7, Arsalan Khan8 and Khudija Ghani9
ABSTRACT
This study was conducted to detect the effect of inorganic selenium on the liver enzymes, kidney function and changes in biochemical parameters of broiler chicken under dexamethasone (DEX) induced stress. The day old, two hundred (n=200) chicks were bought from commercial hatchery and randomly divided into five groups with eight replicas(n=5) in each group. Broiler chickens were fed with starter and finishing commercial corn based basal diet (BD) with different doses of inorganic Selenium (Se) powder. Group-A was kept negative control provided with BD twice a day. Group-B was positive control group fed with 15mg DEX/kg twice/day. The Group-C was fed with BD+0.2mgSe+15mg DEX/kg. Similarly, Group-D was fed with BD+0.3mgSe+15mgDEX. Group-E was fed with BD+0.4mgSe+15mg DEX/kg. Two chicks were randomly slaughtered from each replicate and blood glucose level, kidney function and liver enzyme were examined. After slaughtering, the collected blood was centrifuged for 15 minutes at 1000 rpm for serum analysis and anticoagulant added tube for whole blood. The serum was stored at -20°C until further analysis. Biochemical parameters were measured through commercial kits. The result of the current study showed that the values of Alanine transaminase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP) were higher significantly (P≤0.05) in negative control group (B) as compared to the group C, D and E. The level of uric acid, creatinine and blood urea were significantly higher (P≤0.05) in group A (negative control) (P≤0.05) as compared to the group B (positive control) C, D, and E. The blood cholesterol level and low-density lipoprotein (LDL) in group B (positive control) was significantly higher (P≤0.00) than the group A (negative control) and Se supplemented group C, D and E.
To share on other social networks, click on any share button. What are these?
