Entomopathogenicity and Modeling Aptness of Fungi (Beauveria bassiana and Metarhizium anisopliae) against Pulse Beetle, Callosobruchus chinensis L. (Bruchidae: Coleoptera)
Entomopathogenicity and Modeling Aptness of Fungi (Beauveria bassiana and Metarhizium anisopliae) against Pulse Beetle, Callosobruchus chinensis L. (Bruchidae: Coleoptera)
Wasim Javaid1, Farid Asif Shaheen1*, Farah Naz2 and Muhammad Usman Raja2
ABSTRACT
Aptness of entomopathogenic fungi (EPF), Beauveria bassiana and Metarhizium anisopliae was evaluated to manage pulse beetle (PB), Callosobruchus chinensis by using different fungal concentrations of 1×106, 1×107 and 1×108 spores/ml in stored chickpea grains. Highest mortality (90%) of PB was observed in 72 hours through application of 1×108spores/ml of M. anisopliae compared to B. bassiana with 60% mortality. On 24 hours of exposure, minimum mortality (4%) in PB was recorded in grains treated with 1×106 spores/ml of B. bassiana. In M. anisopliae, the concentrations of 1×108 after 24 hours, 1×107 after 48 hours and 1×107 after 72 hours showed significantly similar results with mortality around 55%. Results proved M. anisopliae as more virulent against PB than B. bassiana and fungal effectiveness against this pest was directly proportional to its concentration as well as exposure time. For effective management of C. chinensis, 1×108 spores/ml of M. anisopliae and B. bassiana is recommended for safer storage of chickpea, pulses and grains.
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