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Morpho-Anatomical Alterations in Asthama Plant (Euphorbia hirta L.) in Various Regions of Faisalabad, Pakistan

Morpho-Anatomical Alterations in Asthama Plant (Euphorbia hirta L.) in Various Regions of Faisalabad, Pakistan

Shahid Ali Khan1*, Farooq Ahmad1, Muhammad Zubair Akram2, Yan Tongyu3, Fatima Urooj1, Kamran Ahmad1, Saeed Ullah4, Sharmeen Zulfiqar5, Tahira Batool6, Aqsa Sarwar1, Yaqoob Sultan7 and Samreen Nazeer8*

1Department of Botany, University of Agriculture, Faisalabad, 38000, Punjab, Pakistan; 2School of Agricultural, Forest, Food and Environmental Sciences, University of Basilicata, Potenza 85100, Italy; 3Department of Life Sciences, Northwest Agriculture and Forestry University, China; 4Shandong Provincial Key Laboratory of Plant Stress, Shandong Normal University, Jinan, China; 5Department of Chemistry, Superior University Lahore, 54000, Punjab Pakistan; 6Department of Botany, Bahauddin Zakariya University, Multan, 60000, Punjab, Pakistan; 7Department of Grass Breeding, Lithunian Reseach Center for Agriculture and Forestry, 58344, Kedainiur, Lithunia; 8Department of Food and Drug, University of Parma, 43124 Parma, Italy.

 

*Correspondence | Shahid Ali Khan and Samreen Nazeer, Department of Botany, University of Agriculture, Faisalabad, 38000, Punjab, Pakistan; Department of Food and Drug, University of Parma, 43124 Parma, Italy; Email: [email protected], [email protected]

ABSTRACT

Euphorbiaceae is the sixth largest family amongst the Anthophyta class of plants. it consists of 340 genera and 9000 species and is cosmopolitan distributed throughout the world. The habits of plants are annuals, perennial herbs, shrubs, and trees. The identifying characteristic feature of plants in this family is the formation of milky latex, because of their succulent nature; they can tolerate or resist water deficit conditions. Different ecotypes of this genus were collected from different areas of Faisalabad region. Different species of this genus Euphorbia hitra was collected by random collection method for morpho-anatomical studies of roots stems, and leaves. After collection, samples were handled carefully and preserved in plastic bottles for sectioning 70% ethanol, and 30% water was used to prepare the solution. The freehand sectioning technique was used for section cutting of roots, stems, and leaves. Permanent slides were prepared after staining. Moreover, the data were subjected to one-way ANOVA (Analysis of Variance) to study morpho-anatomical features of Euphorbia hitra species from Faisalabad regions. This study provided evidence about morphological and anatomical modifications of Euphorbia hitra in response to various environmental fluctuations. Anatomical adaptations such as increased root epidermal and endodermal thicknesses, greater stem area, and large cortical region thickness and cell area play a significant role in different habitats. In conclusion, Euphorbia hitra showed very specific modifications in morphological and anatomical attributes that reveal their ecological success in a variety of habitats.

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Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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