Critical Field Appraisal for the Use of Bovine Tuberculosis’ Antibody Detecting-Serodiagnostics
Critical Field Appraisal for the Use of Bovine Tuberculosis’ Antibody Detecting-Serodiagnostics
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
ABSTRACT
Bovine tuberculosis is a worldwide disease that causes great economic losses to the dairy industry and constitutes a serious human public health hazard. Development of a wide range of serodiagnostic assays for mycobacterium has been an area of interest through the past two decades as a rapid, accurate, and easy-to-be-used and interpreted diagnostic kit as well as to be a competitive alternative to the conventionally established expert-dependent techniques. The current work’s main objective is to evaluate the reliability of two widely distributed rapid kits for as a suitable substitution for the gold standard bovine tuberculosis diagnostic techniques. IQRT Anigen Rapid Bovine TB Ab kit and Ubio quick VET Bovine Tuberculosis Antibody kit were used in testing 3750 dairy cattle. The results obtained were compared with the ELISA, bacteriological examination, and tuberculin skin testing. By the tuberculin test, 69 (1.8%) animals reacted positively, from which 51 animals (73.9%) showed visible lesions on postmortem examination while the remaining 18 animals (26.1%) showed no visible lesions. Using bacteriological examination of tissue specimens, 48 out of the 69 positive tuberculin reactors (69.6%) revealed successful M. bovis isolation. Using ELISA on serum samples collected prior to skin testing from the positive tuberculin reactors, only 26 cases (37.7%) were positive. The IQRT Anigen lateral flow kit showed only 21 positive cases, 43.8% of 48 bacteriologically identified cases, and 30.44% out of the 69 tuberculin-positive cases. The Ubio quick VET kit has detected zero% of bovine tuberculosis-positive cattle. Using bovine tuberculosis rapid kits alone may be unreliable for the detection of tuberculosis-infected cattle.
Keywords | Bovine tuberculosis, tuberculin skin test, lateral flow kits for antibody detection, ELISA, Rapid diagnostics, dairy industry, Human health hazards, Granulomatous diseases
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February 2023
Vol. 11, Iss. 2, Pages 189-354
