TGF-β Mediates the Role of P53 and microRNA-Related Signaling Pathways in the Pathogenesis of Diabetic Nephropathy in Rats
TGF-β Mediates the Role of P53 and microRNA-Related Signaling Pathways in the Pathogenesis of Diabetic Nephropathy in Rats
Yonghong Ma1, Jinlan Ma2, Qifu Zhang3, Huajie Zou1 and Shenghua Ma1*
ABSTRACT
The objective of this study was to study the relationship between TGF-β-mediated P53 and microRNA-related signaling pathways and the occurrence and development of diabetic nephropathy. The sample of rats was randomly assigned to a control group and an experimental group, and a rat model of diabetic nephropathy was established. After the establishment, HE staining, immunohistochemical staining and real-time quantitative PCR were performed to compare the groups. The situation after the successful model creation; observe the two groups of TGF-β1, P53, microRNA103 and microRNA105 mRNA expression determination and TGF-β1 and P53 protein expression levels. The success rate of the experimental rat model was 100%; the expression levels of TGF-β1, P53, and microRNA105 mRNA in the experimental group were significantly higher than those in the control group, and the difference was statistically significant (P<0.05), but the microRNA103 content of the groups was compared. The difference was not statistically significant (P>0.05); after modeling, the expression levels of TGF-β1 and P53 protein in the experimental group were significantly higher than those in the control group (P<0.05). It was concluded that TGF-β-mediated P53 and microRNA-related signaling pathways can coordinate with each other and jointly promote the occurrence and development of diabetic nephropathy.
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