The Effect of Time Interval Differences Between the Prostaglandin F2α Injection and Semen Collection on the Improvement of Spermatozoa and Testosterone Concentrations in Aceh Bull
The Effect of Time Interval Differences Between the Prostaglandin F2α Injection and Semen Collection on the Improvement of Spermatozoa and Testosterone Concentrations in Aceh Bull
Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*
ABSTRACT
The injection of prostaglandin F2 alpha (PGF2α) has been adopted as an alternative to improve sperm quality. The difference in sample collection time after administration is assumed to have a positive effect on testosterone concentration. This research aimed to determine the effect of PGF2α injection and the optimal time for sample collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into two treatments. In treatment 1 (P1), the bull received 25.0 mg PGF2α (Lutalyse™, dinoprost, tromethamine) 1 hour before semen collection, while in treatment 2 (P2), the semen collection was carried out 2 hours after PGF2α administration. The treatment was performed five times in alternate weeks. Semen collection was performed using an artificial vagina once a week for ten weeks. Testosterone concentrations were analyzed using the enzyme-linked immunosorbent assay (ELISA) method. The average spermatozoa volume (mL); concentration (106 cells/mL); and testosterone concentration (ng/mL) in P1 vs. P2, were 4.82±1.93 vs. 4.92±2.45; 889.0±250.50 vs. 721.0±246.25 (P<0.05); and 8.352.34 vs. 9.60±2.24 (P>0.05), respectively. It is concluded that the PGF2α injection 1 hour prior to the collection was able to increase the spermatozoa concentration compared to the 2 hours of treatment, while the value obtained for testosterone was not affected.
Keywords | Aceh bull, ELISA, PGF2α, Sperm, Testosterone, Time interval
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February 2024
Vol. 12, Iss. 2, pp. 194-391
