Assessment of Genotoxicity in Lymphocytes of Active and Passive Cigarette Smokers Attenuated with Green Tea
Assessment of Genotoxicity in Lymphocytes of Active and Passive Cigarette Smokers Attenuated with Green Tea
Saleha Gul1,2, Muhammad Khisroon2, Ajmal Khan2, Attaullah1, Saira Gul3 and Gul Nabi Khan1,4,*
ABSTRACT
The well-reported carcinogens in tobacco smoke are polycyclic aromatic hydrocarbons and nitrosamines cause the production of DNA adducts and leading to lung cancer. However, the adverse effects of cigarette smoke on other tissues are largely unknown. We explored the genotoxic effects of tobacco smoke on peripheral lymphocytes of active and passive smokers. Blood samples were isolated from 100 males’ including 25 active-, 25 passive- and 50 non-smokers. Alkaline comet assay was done and classes were defined on the basis of comet tail length. Significant differences were found in total comet score (TCS) among different groups (p˂0.05). The TCS value of active smokers were significantly increased with other narcotics like charas and snuff (p˂0.05). The passive smokers using snuff were having significantly higher TCS values then the control (p˂0.05). Reduction in TCS values of active smokers using green tea on daily basis were statistically significant (p˂0.05). Taking together, our results indicate that tobacco smoking highly induces DNA damage in blood cells. Additionally, green tea significantly reduces the toxic effects of smoke which is a hope to rescue tissue exposed to smoke toxins.
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