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Purification and Bioactivity of Placental Lactogen from Water Buffalo, Bubalus bubalis

Purification and Bioactivity of Placental Lactogen from Water Buffalo, Bubalus bubalis

Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 

1Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54594
2School of Biological Sciences, University of the Punjab, Lahore 54594

*      Corresponding author: 2amtuljamilsami@gmail.com

ABSTRACT

Mammalian placental lactogen, also known as chorionic somatomammotropin is crucial in controlling the growth of mammary tissue during pregnancy and its release during pregnancy accounts for increased mammogenesis. Bubaline placenta was obtained and processed for extraction of extracellular proteins at pH 9.5 using Tris-Glycine buffer. Placental lactogen was purified by gel filtration chromatography using Sephadex-G100 and ion exchange chromatography on DEAE- Sepharose, employing a linear salt gradient. SDS-PAGE was used to monitor the purification steps, and protein band was located by immunoblotting technique. Bubaline placental lactogen was purified to homogeneity and showed a molecular weight of about 30 kD. Purified placental lactogen was administered to male albino mice for up to eight weeks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to controls when placental lactogen was applied. Increase weight gain for visceral tissues like kidney, liver, muscle and lungs was also observed, as compared to control subjects. It was also recorded that the exogenous supply of bubaline PL reduces fat cells in the hypodermal layer of skin.

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Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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