Evaluation of Oil Vaccines of Avian Influenza H5 Virus in Layer by Enzyme-Linked Immunosorbent Assay
Evaluation of Oil Vaccines of Avian Influenza H5 Virus in Layer by Enzyme-Linked Immunosorbent Assay
Hasan Muwafaq Ali*, Raed Hussain Salih Rabee
ABSTRACT
Highly contagious avian influenza illness is caused by high-path and low-path flue viruses. Chicken vaccinations are intramuscular or subcutaneous injections that reduce illness severity and transmission. To stimulate the immune system, large-scale immunization campaigns may utilize live-attenuated vaccines. Aim of study: This research aimed to assessing the effectiveness of oil vaccines against the H5 virus of avian influenza using an enzyme-linked immunosorbent test in layers. After inoculation with the killed oil vaccine against influenza were tested for H5 immunity in Iraqi Layers hens (LOHMAN Brown). Four treatment groups of 192 chicks were immunized at 1 to 210 days. AI titration by enzyme-linked immunosorbent assay was performed in blood serum to determine immunity. First-day of measurements, ELISA titer was similar across research groups. Significant changes were seen 30 days post-intervention, with MEVAC leading (883.17 ± 173.94). At 50 days post-intervention, MEVAC group had the highest level (2767.33 ± 445.10). At 70 days post-intervention, MEVAC group had the highest level (3269.00 ± 244.67). At each time point assessed, the MEVAC group had the highest ELISA values (90 days 5671.00 ± 330.52; 210 days 10573.75 ± 775.74). Results indicated that the vaccine consistently outperformed other groups in ELISA testing across all evaluated intervals. This indicates a high and prolonged vaccination immunological response.
Keywords | Oil vaccines, Avian influenza, H5N1, H5N8, Enzyme-linked immunosorbent assay
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November 2024
Vol. 12, Sp. Iss. 1
