COMPARATIVE EFFICACY OF ORGANIC SOLVENT FRACTIONS OF LEAF EXTRACT OF HEMP AGAINST Aspergillus versicolor
COMPARATIVE EFFICACY OF ORGANIC SOLVENT FRACTIONS OF LEAF EXTRACT OF HEMP AGAINST Aspergillus versicolor
Iqra Haider Khan1, *Arshad Javaid1 and Nadeem Shad1
ABSTRACT
The fungus Aspergillus versicolor is generally found on food products and produces
sterigmatocystin, a carcinogenic and hepatotoxic mycotoxin. This study reports the
usefulness of polar and non-polar fractions of methanolic extract of hemp (Cannabis sativa
L.) leaves against A. versicolor. Dried leaves of hemp were soaked in methanol for two
weeks. The material was filtered and the methanol was evaporated on a rotary evaporator.
Thereafter, water was added to the residues and the aqueous mixture was successively
partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. Different concentrations
of each fraction were prepared which ranged from 1.562 to 200 mg mL-1. Antifungal activity
was carried out in malt extract broth medium. In general, all the concentrations of the four
organic solvent fractions significantly controlled the growth of A. versicolor. There were 71–
100%, 59–100%, 65–100% and 69–100% decline in the biomass of A. versicolor due to nhexane,
chloroform, ethyl acetate and n-butanol fractions, respectively. It is concluded that
different fractions of leaf extract of C. sativa have remarkable potential in controlling growth
of A. versicolor.
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March 2021
Vol. 27, Iss. 1
