Human Papillomavirus in Spontaneous Abortion
Human Papillomavirus in Spontaneous Abortion
Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal and Gehn Allam
ABSTRACT
First trimester spontaneously aborted products of conception were obtained from 100 Egyptian women. Placental tissue specimens from 100 women of full term normal vaginal deliveries were used as a control group. Their age ranged from 17-42 years (mean = 29.31). The age at marriage lies between 16-36 years. All samples were tested for HPV-DNA by polymerase chain reaction (PCR) amplification using one pair of general primers which allowed for the amplification of HPV types 16, 18, 31, 33, 52, & 58. These primers were designed to amplify E6/E7 gene junction sequences. Twenty nine out of 100 (29%) samples of aborted products of conception were positive for HPV E6/E7 sequences. In comparison. only one of the placental tissue specimens was positive. All the specimens were positive for ꞵ-globin DNA. Positive PCR products were confirmed and subtyped by restriction endonuclease enzyme digestion. Seventeen were type 16. eight were type 18 and four were non-typeable. The highest rate of HPV-DNA positivity was found in patients with age less than 25 years, and in patients with young age at marriage (≈ 20 years). High parity and use of hormonal contraceptives were found to be associated with high frequency of HPV positivity in aborted patients. On conclusion. human Papillomavirus types 16 and 18 might have a role in the occurrence of the first trimester spontaneous abortion among Egyptian women.
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