In vitro: Osmotic potential for virus elimination and preservation of infected banana shoot tip
In vitro: Osmotic potential for virus elimination and preservation of infected banana shoot tip
El-Dougdoug K.A.1, Sofy A.R.2, Rehab A. Dawoud3 and Korkar H.M.4
ABSTRACT
Osmotherapy of virus infected plant materials is a new method for virus
eliminationbased on osmopreservation techniques. Osmotherapy wasapplied for
eliminationbanana bunchy top virus(BBTV) through preservation of shoot tips on
MS media containing high sucrose concentration at low temperature. Shoot tips and
subculture 1st of banana plantlets were preservedsuccessfully for 12 months on
storage MS medium supplemented with 40, 50, 60 and70 gL-1 at 10, 15 and 20ᴼC.
The highest survival percentage 100% with recorded with 40 and 50gm sucrose
concentrations at 10, 15 and 20°C while 70 gL-1at 20°C recorded the lowest
survival percentage, 70.5% sucrose at 12 months. The lowest shoot length and roots
number were recorded with 40 and 50gm sucrose conc.especially at 15°C for 12
months will higher shoot length were recorded with 60 and 70% sucrose at 15°C.
All survival shoot tips at 1st subculture recultured on fresh modified MS media
revealed viability and resumed growth within three weeks. These plantlets were
tested against BBTV by DAS-ELISA. The highest plantlets virus free percentage
95 and 100% were recorded with 60 and 50 gsucrose concentration at 15°C,
respectively. The results of shoot tips virus tested were confirmed by DAS ELISA.
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December 2013
Vol. 10, Pages 1-171
