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Comparative Study between ELISA results and rRT-PCR results for Equine Arteritis virus in Thoroughbred and Foreign Horse Breeds in Egypt

Comparative Study between ELISA results and rRT-PCR results for Equine Arteritis virus in Thoroughbred and Foreign Horse Breeds in Egypt

Ahmed F. Afify1, Mohamed A. Shalaby2, Ahmed A. El-Sanousi2 and Amal S. Gaber1

1Virol.Dept, Animal Health Research Institute, P.O. Box 264-Dokki, Giza-12618, Egypt
2Virol.Dept, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt, 12211

ABSTRACT

Till now the incidence of equine arteritis virus in Egypt is still unknown and need
more investigations.This initiated the present study to design a protocol for isolation
trials and characterization of equine arteritis virus from field specimens. A total of
548 samples were collected from governmental and private studs of Arabian horses
and also foreign horse breeds, including 540 serum samples, among these, 4 EDTAblood
and 4 semen samples. Serologically, indirect ELISA was performed on 540
serum samples. 130 samples were clearly strong positive at a percentage of 24%.
Molecular detection of EAV genome was performed only on 8 selected semen and
EDTA-blood samples which were giving highly distinct positive reaction in the
indirect ELISA. All samples were found negative to the presence of EAV genome by
real time RT-PCR. Trials of isolation on different types of cell cultures were done on
the 4 described semen samples; RK-13, VERO-1008, BHK-21 AND MDBK cell lines
were used in these trials. Six blind serial passages were done using the described
samples; all samples were found negative for the presence of CPE characteristic to
EAV. All sixpassages of all samples on RK-13 cells were re-tested using rRT-PCR
to confirm absence of EAV, thus assuring the absence of EAV in Egypt or at least in
areas from which clinical specimens were collected..

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Journal of Virological Sciences

July

Vol. 3, Iss. 1

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