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Correlation of Polymorphism of AQP7 Gene with the Sperm Cells Quality Traits of the Polish Holstein Friesian Bulls

Correlation of Polymorphism of AQP7 Gene with the Sperm Cells Quality Traits of the Polish Holstein Friesian Bulls

Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7

1Department of Environment, Animal Hygiene and Welfare, Wrocław University of Environmental and Life Sciences, Chełmońskiego 38C, 51-630 Wrocław, Poland.
2Department of Genetics, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology in Szczecin, Piastów Avenue 45, 79-311 Szczecin, Poland.
3Department of Ruminant Sciences, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland.
4Regional Ministry for the Rural Environment, Xunta de Galicia, Spain.
5Escola Superior Agrária do Instituto Politécnico de Viana do Castelo, Rua D. Mendo Afonso, 147, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
6Veterinary and Animal Research Centre (CECAV), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal
7Mountain Research Centre (CIMO), Instituto Politécnico de Viana do Castelo.
 
* Corresponding author: ewa.czerniawska-piatkowska@zut.edu.pl

ABSTRACT

The purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.

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Pakistan Journal of Zoology

December

Vol. 54, Iss. 6, Pages 2501-3000

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