Identification and Isolation of Bacterial Strains and Study of their Ability to Degrade Trimethoprim in the Wastewater of some Kirkuk Hospitals
Identification and Isolation of Bacterial Strains and Study of their Ability to Degrade Trimethoprim in the Wastewater of some Kirkuk Hospitals
Tawoos Mohammed Kamel Ahmed1*, Khalid Mahmood Yosif1, Sameerah Faydhallah Mohammed2
ABSTRACT
The current study aimed to identify and isolate bacterial strains that can degrade pharmaceuticals, which will help reduce contamination. From April 2023 to April 2024, 185 samples were taken monthly from three locations, the effluents of Kirkuk City’s Hospitals. Kirkuk Teaching Hospital, the Pediatrics Hospital, and the Maternity Hospital. Typically, sampling began at nine in the morning and ended at twelve. Water samples were taken from surface water at a depth of 30 to 40 cm using autoclaved amber bottles that had been pre-washed twice with water samples before being filled. The results demonstrated two categories of isolation: mixed bacterial isolates (78; 70.9%) and single bacterial isolates (32; 29.1%). Proteus mirabilis, Escherichia coli, Staphylococcus, Enterococcus, Streptococcus, and other isolates had percentages of 8.2%, 31.8%, 19.1%, 35.5%, and 4.5%, respectively. The 16S RNA gene was verified using the multiplex PCR technique regarding the genetic investigation. The existence of a gene was detected by the presence of a single band at a given molecular weight: 232 bp, 1250 bp, 1500 bp, and 791 bp for the 16SrRNA of the marker used for E. coli, Klebsiella spp., P.mirabilis, and Staphylococcus spp. Regarding biodegradation, none of the examined microorganisms could break down trimethoprim, and it was clear that the drug exhibited a solid resistance to biodegradation . Although TMP is light-sensitive, the abiotic control demonstrated that during the experimentation period, photodegradation did not occur.
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