In vitro efficacy of Tribulus terretris L. & Ziziphus mauritiana Lam. as potential anthelmintics against Haemonchosis contortus
In vitro efficacy of Tribulus terretris L. & Ziziphus mauritiana Lam. as potential anthelmintics against Haemonchosis contortus
ABRAR HUSSAIN1, AQSA KHALIL1, SAIRA ZAHEER1, MUHAMMAD LATEEF2 & MUHAMMAD MANSHA1
ABSTRACT
Ruminants are common victims of helminthic infections which curb the efficient production of livestock. Chemical anthelmintic are used to control parasitic infections but their persistent use develop resistance in parasites. In this connection local plants/ their parts are being used to explore their anthelmintic potential. The aim of the present study was to determine the anthelmintic activity of Tribulus terrestris L. and Ziziphus mauritiana Lam. Whole plant of Tribulus terrestris L. and leaves of Ziziphus mauritiana Lam. were used. The plant materials were dried, ground firmly, macerated in ethanol and then the crude ethanolic extract (CEE) was prepared. Adult motility assay (AMA) was carried out on adult Haemonchus contortus to analyze the anthelmintic efficacy of CEE of the whole plant and the leaves of Tribulus terrestris L and Ziziphus mauritiana Lam. The motility of insects was assessed at concentration of 2, 4, 6 & 8 mg/ml after 2, 4, 6 & 8 hours of post treatment and dead worms were noted. The 8mg/ml concentration of CEE of Tribulus terrestris L. and Ziziphus mauritiana Lam. exhibited 90% and 93% mortality rate, respectively, after 8 hours of post treatment. The positive control, levamisole showed 100% mortality at 0.5mg/ml concentration. It is concluded that Tribulus terrestris L. and Ziziphus mauritiana Lam. possess wormicidal potential and their extracts have anthelmintic activity against Haemonchosis contortus.
To share on other social networks, click on any share button. What are these?
