Prevalence of Hemorrhagic Septicemia and Use of Moringa oleifera and Eucalyptus camaldulensis Extracts against Buffalo Pasteurella multocida Isolates
Prevalence of Hemorrhagic Septicemia and Use of Moringa oleifera and Eucalyptus camaldulensis Extracts against Buffalo Pasteurella multocida Isolates
Barkat Ali Jatoi1*, Amjad Hussain Mirani1, Abdul Latif Bhutto1, Ambreen Laghari2, Abdul Samad Magsi3, Ahmed Sultan Jatoi4, Aneela Sultan Jatoi5, Muhammad Mohsen Rahimoon6, Aarab Khan Lund7, Om Parkash1, Atif Ali Malak8
ABSTRACT
A cross sectional study was conducted to estimate the prevalence rate of Hemorrhagic septicemia (HS) in buffalo at various talukas of district Shaheed Benazirabad (SBA) and to observe the efficacy of drumstick tree (Moringa oleifera) and river red gum (Eucalyptus camaldulensis) plant extracts against Pasteurella multocida. A total of 10 farms of each taluka of Shaheed Benazirabad district (i.e. Nawabshah, Qazi Ahmed, Daur and Sakrand) were visited to examine animals suspected of being infected with HS. A total of 2244 buffaloes and 576 buffalo calves were investigated. Among the suspected animals 100 (blood samples) were cultured, and P. multocida isolates were biochemically characterized. The overall prevalence rate among the observed animals was found to be 11.10% (249 cases) in buffaloes and 9.20% (53 cases) in buffalo calves in the SBA district. The highest prevalence rates were observed in buffaloes (19.59%) and buffalo calves (15.87%) in Qazi Ahmed taluka, followed by buffaloes (15.14%) in Nawabshah and buffalo calves (11.48%) in Daur. The minimum inhibition concentration (MIC) of Moringa oleifera and Eucalyptus camaldulensis was 0.10 and 0.50mg/ml, respectively against P. multocida. The extract of Moringa oleifera significantly (P<0.05) inhibited the P. multocida growth by formation of inhibitory zone diameter 18.33, 15.33, 14.00, 13.00, 10.67 and 9.33mm at concentration level of 2.50, 2.00, 1.50, 1.00, 0.50 and 0.10mg/ml, respectively in contrast to that of Eucalyptus camaldulensis where the zone diameter was 13.00, 9.67, 9.33, 8.33 and 8.33mm at concentration 2.50, 2.00, 1.50, 1.00 and 0.50mg/ml, respectively. The average inhibition zone formed by Moringa oleifera (13.55mm) was noted markedly higher (P<0.05) than that formed by eucalyptus camaldulensis (8.75mm). It could be concluded that the Moringa oleifera plant extract found to be efficient against P. multocida compared to that of Eucalyptus camaldulensis.
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