Specific PCR-based Marker for Identification of Common Carp ‘Hainan’ in China
Specific PCR-based Marker for Identification of Common Carp ‘Hainan’ in China
Muhammad Younis Laghari1,2, Zixia Zhao1, Yiwen Gong3, Punhal Lashari1,2, Shangqi Li1, Jian Xu1 , Ateeque Rahman Khooharo4, Jiongtang Li1,* and Yan Zhang1*
ABSTRACT
The common carp (Cyprinus carpio L.) is one of the most important aquaculture species in the world. In China, due to the geographical isolation of Hainan island from the mainland, the genetic diversity in Hainan island is different to that in the mainland. One of fifty sequences isolated from SINE element was polymorphic, including six haplotypes with an average sequence divergence of 11.03% (SE = 0.78%). Four haplotypes (MF177501-MF177504) were shared in these four population. However, two specific haplotypes (MF177499-MF177500) were only observed in Hainan population. To simplify procedure and improve effectiveness of identifying the Hainan population, one specific PCR-based marker acted as INDEL marker was developed and then 120 samples were used as templates for PCR validation. The specific marker will facilitate the assessment of genetic diversity and selective breeding programs for Hainan common carp.
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June 2020
Vol. 52, Iss. 3, Pages 825-1224
