Effect of Different Conditions of Citric Acid and Acetic Acid Decontamination against Listeria Monocytogenes in Lettuce
Effect of Different Conditions of Citric Acid and Acetic Acid Decontamination against Listeria Monocytogenes in Lettuce
Wan Norhafizah Wan Azman and John Yew Huat Tang*
ABSTRACT
The purpose of this study was to assess the effectiveness of various citric and acetic acid conditions against L. monocytogenes in lettuce, as well as to ascertain the overall reduction of L. monocytogenes by various decontamination procedures. Before decontamination process, the sample was inoculated with L. monocytogenes and stored in the chiller for 24 h. Different concentrations of acetic and citric acid (0%, 0.5%, 1.0%, 1.5%, and 2.0%), as well as varied times (0, 15, and 30 min) and physical applications (agitation and without agitation) were used to clean the sample. After being incubated for 48 h at 30oC, the numbers of L. monocytogenes was counted. It has been demonstrated that at a concentration of 1.5%, citric acid is efficient in eliminating L. monocytogenes without agitation. With a citric acid (1.0%), the effectiveness of citric acid in killing L. monocytogenes is greatly enhanced by the use of physical forces. The duration of the citric acid treatment was also found to have a major impact on the elimination of L. monocytogenes in lettuce. Citric acid application for 30 min was shown to be significantly more efficacious than that for fifteen minutes (p<0.05). On the other hand, at a concentration of 2.0%, the results indicated that acetic acid is highly effective in eliminating L. monocytogenes without the need for physical application. However, with agitation, it exhibits a notable decrease at the minimal concentration of 0.5%. The amount of L. monocytogenes does not, however, significantly change when the period is extended from 15 to 30 min. To summarize, when physical force is applied, acetic acid is more effective than citric acid; nevertheless, without agitation, the data demonstrate that citric acid is more effective than acetic acid.
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August 2024
Vol. 40, Sp. Iss. 1
