Histopathological and Micrometric studies of Diazinon exposure on Thyroid and Parathyroid Tissues in Mice
Histopathological and Micrometric studies of Diazinon exposure on Thyroid and Parathyroid Tissues in Mice
SYEDA NADIA AHMAD1, MEHWISH NASIR1, AQSA NAUREEN1, SHAGUFTA ANDLEEB4, KAUSAR RAEES3, TAHIR ABBAS2, ASMA YOUNIS1 & KHAWAJA RAEES AHMAD*1
ABSTRACT
Effects of Diazinon (DZ) were studied at 0, 2.5, 5, 10 and 20 mgkg-1 in adult male mice (10 in each dose group) for thyroid and parathyroid histopathology. Tissues were recovered following 48 h of DZ treatment. Micrometric data in terms of follicular size of thyroid, cellular, nuclear sizes of the follicular and chief cells were obtained from the histological sections. Statistical analysis has shown significant (p<0.001) variations in mean follicular size of thyroid indicating dose dependent depletion of colloid (thyroglobulin). Mean Cross-Sectional Area (MCSA) of follicular cells showed increase in DZ groups as compared to the control with maximum rise at 2.5 and 5mg exposure levels while the MCSA of their nuclei remained more-or-less constant. Statistical analysis has shown significant variation (p<0.05) in MCSA of the follicular cells among the groups. Histopathological observations revealed dose dependent apoptotic changes in follicular cells and chief cells of parathyroid. Chief cells MCSA showed slight variations. Nuclear MCSA of the chief cells showed significant (p<0.001) dose dependent decline. The present findings suggest that DZ is toxic to endocrine cells of thyroid and parathyroidat 5mgkg-1 or more, in mice, bringing about characteristic histopathological and micrometric changes in these tissues.
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