Biological, serological and inhibition activity of Tomato spotted wilt virus isolated from tomato plants in Taif region, Saudi Arabia
Biological, serological and inhibition activity of Tomato spotted wilt virus isolated from tomato plants in Taif region, Saudi Arabia
Manal A. El-Shazly1. M. I .Kobeasy2and Sarah H . Altalhi3
ABSTRACT
Tomato spotted wilt virus (TSWV) has been isolated from naturally infected tomato plants collected from Taif governorate, Saudia Arabia for the first time. Observed symptoms circumvented mosaic, curling, bronzing and/or purpling, chlorosis and necrotic spot on the leaves. Disease symptoms of infected fruits produced from inoculated healthy tomato seedling showing discoloration, faint concentric rings, necrotic and chlorotic spot on mature tomato fruits. The virus was biologically purified from a single local lesion formed on Chenopodium amaranticolor Caste & Reyn. The isolated virus was identified on the basis of symptomatology, transmissibility, host range and serological tests by indirect –enzyme linked immunosorbent assay (Indirect- ELISA) and dot blotting immunobinding assay (DBIA) using an induced antiserum for TSWV. All the five tested tomato cultivars were found to be susceptible when mechanical inoculated under greenhouse conditions. Wide variations of symptoms were found between cultivars. Super strain and super merman were found to be more susceptible than any other cultivar tested which showing 90% infection. Basil essential oil and ethanolic extract of Plantago major leaves was used as antiviral to reduce the infection with TSWV at three concentrations levels (500,1000 and 2000 μg/ml and 50,125,250 μg/ml, respectively).Viral infection was reduced by using the highest concentration of Basil essential oil and ethanolic extract reached to 45.99% and 63.82%, respectively. Different treatments with antiviral compounds decreased the content of total soluble sugars, titrable acidity also increased the lycopene and ascorbic acid in tomato fruits compared with infected plants.
To share on other social networks, click on any share button. What are these?
