Allelic Diversity of the Endangered Taro White Cattle Population from Bali using BoLA Microsatellite Loci
Allelic Diversity of the Endangered Taro White Cattle Population from Bali using BoLA Microsatellite Loci
I Nyoman Sulabda1*, I Nengah Wandia2, I Ketut Puja3
ABSTRACT
Taro white cattle is an indigenous and endangered cattle population in Taro village, Bali and only inhabiting restricted areas in forests of Taro village. Current population are 57 head. To provide basic information concerning allele and locus in BoLA gene, the BoLA microsatellite loci (DRB3 and BM1815) were used to evaluate allel diversity using 18 samples of Taro white cattle. Genetic variability was low, with observed heterozygosity and expected heterozygosity for locus BM1815 was 0.778 and 0.51, and for DRB3 was 0.556 and 0.478, respectively. Moreover, the polymorphic information content (PIC) for these two microsatellite was 0.397 for BM1815 and 0.413 for DRB3. The Taro white cattle showed low level of genetic diversity. This result would be used as an initial guide for conservation decisions in future for survival of this valuable genetic resource. The alleles identified from the present study should be further confirmed by sequencing.
Keywords | Taro white cattle, Microsatellite, BoLA, Allel, Heterozygosity
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