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Ce-doped Nanobioactive Glass/ Collagen/ Chitosan Composite Scaffolds: Biocompatibility with Normal Rabbit’s Osteoblast Cells and Anticancer Activity Test

Ce-doped Nanobioactive Glass/ Collagen/ Chitosan Composite Scaffolds: Biocompatibility with Normal Rabbit’s Osteoblast Cells and Anticancer Activity Test

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

1The Healthy Chemistry Department, Center Public Health Laboratory, Ministry of Health, Egypt; 2Glass Research Department, National Research Centre, Dokki, 12622, Giza, Egypt; 3Department Pathology, Tissue Culture and Cytogenesis Unit, National Cancer Institute, Cairo University, Egypt; 4Department of biochemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.

*Correspondence | Mohammad Mohamoud Farag, Glass Research Department, National Research Centre, 33 El-Behouth Str., Dokki, 12622, Giza, Egypt; Email: mmfaragnrc@gmail.com

ABSTRACT

This research aims to evaluate cerium-doped nanobioactive glass/collagen/chitosan composites scaffolds with osteoblast mineralization of normal rabbit bone marrow mesenchymal stem cells (rBM-MSCs) and cancer osteosarcoma cells. The non-cellular in vitro bioactivity test was performed in simulated body fluids for periods 1, 3, 10, 20 and 30 d by measuring the calcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells derived from isolated rBM-MSCs by flowcytometric analysis was studied by histochemical staining with Alizarin Red and von Kossa to confirm the osteogenic differentiation process. Also, cell viability assay by MTT was used to measure the number of viable osteoblast cells cultured with scaffolds extracts. Also, the antitumor activity of the scaffolds was studied against cancer osteosarcoma cell lines using Sulforhodamine B (SRB) assay. The results showed that addition of cerium-doped nanobioactive glass to the composite scaffolds was triggered an increase in cell growth, proliferation and mineralization markers of osteoblast cells that increased with time as the highest concentrations of CeO2 innanobioactive glass (sample CL/CH/C10). Cell viability proved also that all scaffolds and their extracts showed proliferation inhibition with time < 25% reference to final cell number of control cells. Among the composites, having CL/CH/C5 showed the highest cytotoxic effect and reduced survival rate of osteosarcoma cells to 75.68% after 24 h. The subsequent increase of CeO2 concentration was also effective but its effect was less than CL/CH/C5 sample. Finally, cerium-doped nanobioactive glass/collagen/chitosan composites scaffolds were exhibited good biocompatibility on normal cells and increased cytotoxicity on cancer osteosaroma cells.
 
Keywords
| Bioactivity, Scaffold, Osteogensis, Cerium oxide particles, Mesenchmal stem cells, Osteosarcoma cells

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Advances in Animal and Veterinary Sciences

June

Vol. 12, Iss. 6, pp. 994-1205

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