Serological Detection of Burkhorderia mallei in Equine
Serological Detection of Burkhorderia mallei in Equine
Ali Mohammed Obayes*, Zaid Salah Hussein
ABSTRACT
The current study was conducted to assess the application and demonstration of rapid, sensitive, and specific assays for confirmatory testing and to determine the prevalence of glanders in horses and donkeys. For this reason, a competitive enzyme‐linked immunosorbent assay (cELISA) was applied to determine seropositivity against Burkholderia mallei in clinically confirmed cases of glanders from Baghdad city. Analysis of sera samples collected from horses (n=80) demonstrated that 36 horses were positive with a percentage of 22.5 % positivity rate. Of the donkey (n = 80), a total of 4 cases were identified as positive for glanders with a percentage positive rate of 2.5 %. However, no significant variations (p <0.05) in glanders seropositivity were observed between different sex or age groups. These findings highlight alarming seropositivity in equines and warrant future investigations on the prevalence of active infection.
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