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Comparison of Pregnant Diagnosis in Local Buffalo with a Seed Germination Inhibition Test and Rectal Palpation

Comparison of Pregnant Diagnosis in Local Buffalo with a Seed Germination Inhibition Test and Rectal Palpation

Ferry Lismanto Syaiful1*, Jaswandi Jaswandi2, Mangku Mundana2, Ilham Ilham2, Novirman Jamarun1, Efrizal Efrizal3

1Faculty of Animal Science, Andalas University, Padang, West Sumatra, Indonesia; 2Faculty of Animal Science, Andalas University, Padang, West Sumatra, Indonesia; 3Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang, West Sumatera, Indonesia.
 
*Correspondence | Ferry Lismanto Syaiful, Engineer Professional Program, Faculty of Animal Science, Andalas University, Padang, West Sumatra, Indonesia; Email: ferrylismanto@ansci.unand.ac.id, ferrylismanto5@gmail.com

ABSTRACT

Pregnancy detection is the most essential aspect after insemination, this can increase reproductive efficiency and buffalo birth. This study is aimed is evaluating the use of mung bean seeds on multiple dosages in the growing seeds tests for the accuracy, sensitivity, and phases of local buffalo pregnancy, and detecting buffalo pregnancy quickly, massively, and cheaper. The object of this study was 40 local buffalo post-AI with the criteria of BCS ≥3 and 2-3 parturition. The research medium used was green bean seeds. Then use urine and water samples. The urine samples used in this study were 120 samples. The dosage uses the ratio of mixing urine and water 1:12 mL, 1:14 mL, and 1:16 mL. The variables of this research are accuracy, sensitivity, and the phases of local buffalo pregnancy. This research shows that the level of local buffalo on the days-21; 42; 63 after AI is 7.50%; 55%; and 70%. The accuracy of local buffalo pregnancy is 78.57%. Moreover, the sensitivity of buffalo pregnancy using mung bean seed in multiple dosages of treatment (1:12; 1:14; 1:16mL) on days-21; 42; 63 after AI shows results of 10.71%; 78.57%; 100%. The finding of this research shows that the use of mung bean seed can detect the phases of local buffalo pregnancy with up to 70% accuracy; the sensitivity of buffalo pregnancy is 78.57% and 100% pregnancy accuracy. Buffalo pregnancy test using a seed germination inhibition test can detect pregnancy easily, practically, massively, and more economically.
 
Keywords | Accuracy, Sensitivity, Local buffalo pregnancy, Seed germination inhibition, Rectal palpation, Bio-assay test

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Advances in Animal and Veterinary Sciences

June

Vol. 12, Iss. 6, pp. 994-1205

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