Therapeutic Effect of Heterologous Platelet-Rich Plasma on Third-Degree Burn Wound in Rabbits
Therapeutic Effect of Heterologous Platelet-Rich Plasma on Third-Degree Burn Wound in Rabbits
Kazi Afsana Homayra Orchy1, Mst. Antora Akter1, Nelema Yesmin1, Md. Moshiur Rahman Khan1, Marzia Rahman2, Md. Mahmudul Alam1*
ABSTRACT
The study was conducted on rabbits to evaluate the efficacy of heterologous platelet-rich plasma (hPRP) in the treatment of third-degree burn wounds. A total of 18 circular third-degree burn wounds measuring 10mm in diameter were made in six rabbits and studied under two groups: Group A and Group B. The wounds of Group A were treated topically with freshly prepared hPRP gel twice a week. The control rabbits received normal saline topically. The wounds were evaluated for 45 days post-burning. Morphological features of wounds such as pain sensation, the development of crusts, exudates and pus, and morphometric assessment like wound contraction were recorded. On the 7th, 14th and 21st days post-burning, biopsies were taken for histopathological assessment. In this study, the morphological and morphometric data revealed that in the animals of group A, wounds were healed up with improved morphological features significantly earlier than those of group B which was confirmed in the histological analysis of biopsy sample. Regarding the liver and kidney functions, the serum levels of liver enzymes (ALT, AST and ALP) and kidney function biomarkers (creatinine, BUN) showed a significant increase in burn patients of the saline-treated control animals, whereas, the animals treated with hPRP exhibited the normal range of these functional biomarkers. On the basis of morphology, histopathology, and biochemical analysis, it can be concluded that PRP, irrelevant of source improves and accelerates the burn wound healing with no adverse effects
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