Effect of Propranolol in Hepatic Blood Flow: A Way to Encounter Phenytoin Induced Hepatotoxicity in Albino Rabbits
Effect of Propranolol in Hepatic Blood Flow: A Way to Encounter Phenytoin Induced Hepatotoxicity in Albino Rabbits
Hina Abrar1*, Hina Yasin1, Hira Naeem2, Rabia Bushra1, Shaukat Mahmood2 and Kaneez Fatima3
ABSTRACT
Phenytoin has proved to be the most effective anti-seizure drug to treat the partial and tonic clonic seizure. However; hepatotoxicity associated with the Phenytoin (PHY) is of great concern in clinical settings. Contrarywise, Propranolol (PRL) is reported to possess the significant hepatoprotective effects owing to the reduced blood flow. Therefore, the present study was aimed to evaluate the hepatotoxicity of PHY alone and in combination with PRL in healthy male rabbits. Post treatment level of various liver enzymes including alanine transaminase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (γ-GT) and bilirubin (BRB) were measured and compared with the normal values. Additionally, histological examination was done through micrometry and scanning electron microscopy (SEM) to estimate the intensity of hepatotoxicity induced by PHY and PHY with PRL. Animals received PHY alone treatment showed a significant elevation of liver enzymes than control and combination. Conversely, the lower level of serum ALT, ALP, γGT and BRB were found when administered PRL with PHY (p<0.005). Prominent change in hepatic cells structure and diameter of nucleus were observed (p<0.05). Severe hepatic damage reflected by necrosis, hemorrhage and dilation of sinusoids, inflammation with dilation and congestion of portal vein were obvious in PHY treated group. Combination treatment of PHY with PRL showed normal liver function and identical tissue pattern as control group exhibited. It is concluded that the PRL actually reduces the blood flow to the liver consequently, supply of noxious substance is blocked. Henceforth, the PRL would provide the beneficial effects when co-administered with the hepato-toxic drugs.
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