Inquisition of Malathion Induced Histopathology, Nuclear Abnormalities in Erythrocytes and DNA damage in Hypophthalmichthys molitrix
Inquisition of Malathion Induced Histopathology, Nuclear Abnormalities in Erythrocytes and DNA damage in Hypophthalmichthys molitrix
Saima Naz1*, Moazama Batool2*, Qurat Ul Ain2, Ahmad Manan Mustafa Chatha3, Sheeza Bano2, Sadia Nazir4, Ghulam Abbas5 and Unab Zahra1
ABSTRACT
This study investigates the impact of a newly introduced insecticide, Malathion on the health of Hypophthalmichthys molitrix fish. The fish serve as valuable biomarkers for ecological harm caused by toxins, especially as they are a significant source of protein and easily accessible. The current study focuses on histopathological alterations, nuclear changes in erythrocytes, and DNA damage in fish brain after exposure to Malathion at environmentally relevant concentrations. The research aimed to assess the potential toxicity of Malathion on fish health, utilizing histological examinations and genetic toxicity assessments through micronuclei and Comet assays. Freshwater fish were exposed to Malathion for 18 days under constant pH, temperature and hardness conditions. The study involved sacrificing fish on the 6th, 12th, and 18th days of exposure for tissue and blood sample collection. Histological examinations revealed significant alterations in the fish brain, including necrosis of neurons, cytoplasmic vacuolization, edema, and congestion of neural cells. The severity of these changes increased with higher Malathion concentrations and longer exposure periods. The micronuclei and Comet assays were employed to evaluate genetic toxicities, providing insights into the potential harm posed by Malathion to fish health. The findings underscore the importance of understanding the impact of environmental pollutants on aquatic ecosystems and emphasize the necessity for effective monitoring and mitigation strategies. The observed histopathological alterations and genetic toxicities highlight potential risks associated with Malathion exposure, emphasizing the need for careful consideration of the environmental consequences of new insecticides. The results showed significant morphological changes in red blood cells, severe histological changes and DNA damage in brain tissues of exposed fish. Thus, malathion in water have toxic effects on fish therefore its seepage into aquatic bodies should be carefully monitored.
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