Genetic Diversity and Population Genetics of Cuttlefish Sepiella japonica based on Newly Developed SSR Markers
Genetic Diversity and Population Genetics of Cuttlefish Sepiella japonica based on Newly Developed SSR Markers
Yingying Ye1, Chengrui Yan1, Kaida Xu2, Jiji Li1, Jing Miao1, Zhenming Lü1 and Baoying Guo1*
ABSTRACT
Cuttlefish Sepiella japonica, an economically valuable fishery resource that is considered as one of the four most famous sea products in China, has dramatically declined since the 1990s to near extinction, presumably due to over exploitation and environmental disruption and particularly because of the deterioration of their spawning grounds. In this study, we developed primers for nine microsatellite loci resulting from an enrichment process based on the Wenzhou population. Eight out of nine microsatellite loci were used to analyze population genetic diversity of cuttlefish in China Sea. The results showed high levels of genetic diversity in all populations with highest mean number of alleles and mean effective alleles estimated in population Ningde and lowest in Qingdao. Heterozygosity at species level was high and Fst ranged between 0.105 and 0.271, overall, without signs of inbreeding except for Qingdao population, with Fst = 0.271. The studied populations did not show a clear genetic structure with an average differentiation estimated between populations of 0.014. Knowledge obtained from this study has important implications for the management of existing genetic resources for aquaculture, artificial releasing project and conservation in cuttlefish.
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