Efficacy of Carica papaya Leaves Extract for Treating Thrombocytopenia: An In Silico and In Vivo Study in Rat Model
Efficacy of Carica papaya Leaves Extract for Treating Thrombocytopenia: An In Silico and In Vivo Study in Rat Model
Sony Eka Nugraha1*, Marianne Marianne2, Rony Abdi Syahputra2, Aji Najihudin3, Nikmatul Ikhrom Eka Jayani4, Shaum Shiyan5, Nabila Nabila6,7
ABSTRACT
The aim of this study was to determine the anti-thrombocytopenic activity of the ethanol extract of C. papaya leaves in rats using in silico and in vivo studies. Analysis of phytochemical compounds was carried out using GCMS and qualitative phytochemical screening. Additionally, antioxidant activity was determined by spectrophotometry. A rat model of thrombocytopenia was established by subcutaneous injection of heparin at 2000 IU/kg body weight (BW) for 10 days. After induction, rats were administered C. papaya leaves ethanol extract at various concentrations (50, 100, and 200 mg/kg BW) for 20 days. On day 21, the duke method was used to examine the bleeding time, and the blood clotting time was evaluated using the Lee White method. Blood samples were evaluated for platelet concentration, thrombopoietin concentration, prothrombin time, and active partial thromboplastin time. These results indicated that the C. papaya leaf extract contained various compounds. ABTS antioxidant activity assay showed an IC50 of 45.50 μg/ml. In vivo examination revealed that C. papaya extract has potential anti-thrombocytopenic effects; doses of 100 and 200 mg/kg BW showed significant improvement in the hematology profile from that of the heparin control (p < 0.05). In particular, doses of 200 mg/kg BW have the best potential for the development of anti-thrombocytopenic agents. Furthermore, an in silico study found that the main compound of C. papaya interacts with multiple amino acid residues, indicating stable binding affinity. Finally, it can be concluded that C. papaya has potential as an anti-thrombocytopenic agent.
Keywords | Carica papaya, Hematology, Heparin, in silico, in vivo, Thrombocytopenia
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July 2024
Vol. 12, Iss. 7, pp. 1206-1409
