In vitro culture of water hyacinth (Eichhornia crassipes)
In vitro culture of water hyacinth (Eichhornia crassipes)
Tanvir Hussain
ABSTRACT
The current study was undertaken in 2001 to assess the in-vitro culture potential of water hyacinth by taking different plant-parts (root, stem, leaf, anther, stolon, ovules, embryos/embryos with stem) as explants in different culture media i.e. Murashige & Skooge (MS) medium, Gamborgs B5-salt (B5)and Basal nutrient medium (BNM) alone and in combination with Auxins and Cytokinins. It was found that only the axillary buds gave good response to all media. For callus initiation, same ex-plants and media (alone and in combination with callus initiators) were tried but callus formation was not observed. Aseptically grown young plants were then transferred to hydroponic conditions for maturity. For this purpose MS (Full-strength, 1/2, 1/4), B.N.M and Hooglands solution were used. It was researched out that 1/4 MS (pH 5.8) and B.N.M. (pH 5.8) gave excellent results. The study concluded that water hyacinth can be cultured and established in-situ by using usual techniques and methodologies of tissue culture.
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June 2007
Vol. 57, Iss. 2
