Application of Bioactive Nano-Glass Fiber in Combination with Fresh Platelet-Rich Plasma on the Re-Epithelialization of the Thermal Wound in Rats
Application of Bioactive Nano-Glass Fiber in Combination with Fresh Platelet-Rich Plasma on the Re-Epithelialization of the Thermal Wound in Rats
Reza Esmaealzade Dizaji1, Arasb Dabbagh Moghaddam1*, Arash Ghalyanchi Langeroudi2, Mohamad Foad Heydari3, Seyyed Javad Hosseini Shokouh4 and Ana Shirzad Shahrivar5
ABSTRACT
In this study, topical application of bioactive nano-glass fibers (BNF) loaded with platelet-rich plasma (PRP) was used to examine the effects on a second-degree heat wound and the healing process’ sub-streaming molecular route for re-epithelialization. Model (deep second-degree thermal wound), PRP, and PRP/BNF groups were each randomly assigned a set of animals. Wounds were monitored daily for many days following therapy to see how they were healing. H&E staining and qRT-PCR were used to examine the pathological alterations in wound tissues and to determine mRNA expression of inflammatory and pro-inflammatory cytokines. We found that the PRP/BNF group and the PRP group had faster wound healing rates than the model group at each time point in the current investigation. The same results were corroborated by the pathological score of skin wounds stained with H&E. Researchers found that in comparison to the control group, those in both the PRP/BNF and PRP groups had significantly lower expression levels of the mRNAs for interleukin 1, interleukin 6, and interleukin 10 than those in the untreated group. In contrast, those in the untreated group had significantly higher expression levels of those genes. The PRP/BNF and PRP promote wound epithelization by raising the expression of EGF, VEGF, TGF-, HIF-1, and Integrin 3, while boosting the release of Integrin 1 and other mechanisms, reducing healing time and improving healing quality.
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