Molecular Identification of Several Pseudomonas aeruginosa Virulence Genes
Molecular Identification of Several Pseudomonas aeruginosa Virulence Genes
Baraa Qasim Mohammed1, Asmaa Hamoody Abdullah1, Ahmed Raheem Rayshan2*
ABSTRACT
The investigation was carried out to identify virulence factors activity and to diagnose Pseudomonas aeruginosa from otitis infection of dog and its susceptibility to essential antibiotics. For this purpose, a total of twelve ear swabs were taken from affected canines. The isolates were initially identified based on their morphological characteristics, such as size, color, and shape, as well as by Gram’s staining, biochemical tests, and Vitek2 system confirmation. Pseudomonas aeruginosa was then confirmed by PCR assay. Importantly, all isolates tested positive for the ability to produce the virulence factor hemolysin, a phospholipase enzyme. The 16S rRNA gene was first identified as a virulence gene by polymerase chain reaction study, followed by the identification of the exotoxin T, outer membrane protein I, and phenzaine+ M genes. Three (27.27%) of the isolates were P. aeruginosa carrying exoT genes, four (36.36%) P. aeruginosa isolated carried oprl genes, and three (27.27%) isolates of P. aeruginosa carrying phz+M genes. By using traditional PCR, oprl had the highest prevalence of virulence genes with 504 bp fragments, followed by exoT with 153 bp fragment and phz+M with 128 bp fragment. High levels of significance (P<0.01) were found in all of the study’s components. Finding of the study highlight the prevalence of P. aeruginosa from dog otitis infections and disease associated molecular marker which can guide future design of improved treatments.
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