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Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water

Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water

Amina A. II. and M.K. Amin

Fac. Agric. Zagazig Univ. Dept. Genetics


Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of plaques (turbid and clear) have been observed. The average number of turbid plaques was 2.52 x 109 pfu/ml whereas of clear plaques was 9.96 x 107 pfu/n) l. The phage was able to lysis 13 out of 20 strains of Pseudomonas aeruginosa, this result shows that phage PAI has a broad host range. The phage was able to lysogenized three strains (PAOI, PU21. MAM2) and was also able to release spontaneously from these lysogenic strains. The phage particles which released spontaneously from lysogens were used for transducing different genes from donor ATC43 to recipient ATC 1 14. The phage was able to transduce the same marker with nearly the same frequency. When phage was treated by different temperature, the phage was stable informing plaques with 5, 150C whereas the pfu/ml 6.8, 6.9 x 109, but the ability of phage to form plaques was declined with low and high temperature (1.8 at OOC and 0.18 at 450C x 109 pfu/ml). The ability to transduce antibiotic resistance genes was also influenced. When phage was treated with 5 different Ph values (2, 3, 7, 10, 12), no plaques or transductants were observed at acid Ph values (2, 3). But with Ph7 resulting high titration and subsequently high number of transductants. This study demonstrates that the bacteriophages as a mediated generalized transducing vectors of genetic material play role in natural ecology.

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Journal of Virological Sciences


Vol. 3, Iss. 1


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